Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Extraction optimization of Eucommia ulmoides Oliver and its effect on bone quality in OVX rats

ZhengNan Zhao¹, XiangLong Yang¹, Maoxun Li², Fang Yu¹, HaiDong Liang¹

¹Reparative and Reconstructive Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116023; ²The People's Hospital of Jimo, Qingdao, China.

For correspondence:- HaiDong Liang Email: lianghddlf@sina.com Tel:+8641184671291

Accepted: 25 February 2020 Published: 31 March 2020

Citation: Zhao Z, Yang X, Li M, Yu F, Liang H. Extraction optimization of Eucommia ulmoides Oliver and its effect on bone quality in OVX rats. Trop J Pharm Res 2020; 19(3):623-628 doi: 10.4314/tjpr.v19i3.24

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To maximize the yield of extract from Eucommia ulmoides Oliver and its effect on bone quality.

Methods: Different extraction indices were optimized with response surface methodology (RSM) for maximization of extract yield from Eucommia ulmoides Oliver. Box–Behnken design (BBD) was used to identify the effects of temperature, time, and liquid to solid ratio on extract yield from Eucommia ulmoides Oliver. After 4-week acclimatization, thiry-two rats were randomly assigned to 4 groups (n = 8): group 1 (sham) given vehicle only; group 2 (OVX rats given Eucommia ulmoides Oliver extract at a dose of 4 g/kg; group 3 (OVX + vehicle); group 4 (OVX + EUOE), i.e., OVX rats given Eucommia ulmoides Oliver extract (4 g/kg). Sham rats had intact ovaries. After surgery, the rats received gentamicin intramuscularly for 3 successive days. Two months after surgery, blood and trabecular bones was taken for analysis.

Results: Temperature and liquid-to-solid ratio had marked impact on extract yield from Eucommia ulmoides Oliver, with the best conditions being temperature of 88 °C, time of 137 min, and liquid to solid ratio 16:1. Using these optimized conditions, the maximum yield of extract obtained experimentally (2.53 %) was very close to the predicted value of 2.49 %. There was a good fit between the mathematical model evolved and the data on extract yield. The extract significantly (p < 0.01) increased the Ca and P and Cr levels in OVX + EUOE group compared to those in OVX control. Moreover, the extract significantly (p < 0.01) increased macro-mechanical indices of trabecular bone in OVX+EUOE group, relative to those in OVX control.

Conclusion: The yield of Eucommia ulmoides Oliver extract has been successfully optimized using RSM. The extract exhibited strong effects on bone quality.

Keywords: Optimization, Eucommia ulmoides, BoxÃ¢â‚¬â€œBehnken design, Response surface methodology, Bone loss, Gene